Biosafety

Biosafety manual

• ASU Biosafety manual. 

Bloodborne pathogens 

• ASU Bloodborne Pathogen Exposure Control Plan.

• Blood-borne pathogens fact sheet.

Forms

• Biological purchase or transfer.

• Hepatitis B vaccine consent or declination.

• Lab-specific biosafety training.

Field research

The ASU Field Research Safety Manual, or FRSM, provides guidelines, policies, requirements and resources for ASU personnel to conduct field research safely and successfully. The manual helps ASU personnel minimize risk associated with conducting field research in local, regional, national or international locations. Field sites may include the following areas or other field stations where resources, including emergency assistance, may be limited:

• Coastlines. 
• Construction areas.
• Excavations.
• Mines. 
• Natural reserves.
• Public lands or parks.
• Waterways.
• Wilderness locations.

Environmental Health and Safety intends that the Principal Investigator and supervisory personnel supplement the FRSM information with instructions and guidance about specific practices and procedures unique to fieldwork. The FRSM is an EHS document written in conjunction with the Institutional Biosafety Committee and the Institutional Animal Care and Use Committee. 

• Field research manual.

• Field research safety plan — Appendix A.

Guidance

COVID-19: 

• Computing labs and technology studios:

  • ASU computer lab and technology studio ramp up guidance and checklist.

  • Computer returns disinfection protocol.

• Ice machine user's guidance.

• Reporting.

• Laboratory ramp-up:

  • ASU lab ramp-up checklist.

  • ASU lab ramp-up guidelines.

  • Preventing the spread of respiratory viruses.

Resources:

• ABSL PPE table.

• Animal biosafety PPE requirements.

• ASU computer returns disinfection protocol.

• Autoclave poster.

• Biological waste handling.

• Biosafety PPE requirements.

• Large-scale research fact sheet.

• Lentivirus guidance document.

• Preventing the spread of respiratory viruses.

• Recombinant/synthetic nucleic acid fact sheet.

• Select agents and toxins fact sheet.

• Sharps handling and disposal fact sheet.

• Synthetic nucleic acid guidance.

• Transgenic insects fact sheet.

Standard operating procedures:

• Autoclave manual.

  • Autoclave operation.

  • Autoclave safety video.

• Biological safety cabinet.

  • Biological safety cabinet decontamination.

• Biological safety level two | BSL-2.

• Needlestick and sharps response guide.

• Plumbing decontamination guide.

Researcher

Each Principal Investigator engaged in biological research should have lab-specific information available that includes the following documents in addition to any supplemental materials that will augment the safe and proper handling of biohazardous materials in their laboratory.

New researchers must apply to the Institutional Biosafety Committee in the Office of Research Integrity and Assurance.

1. Every laboratory operating at BSL2 or BSL3 must have a copy of the ASU Biosafety Manual available.

2. Laboratories that work with recombinant or synthetic nucleic acid molecules must have a copy of the NIH Guidelines Research Involving Recombinant or Synthetic Nucleic Acid Molecules available.

3. It is recommended that all approved IBC disclosures be made available to laboratory personnel.

4. EHS performs annual biosafety inspections and provides a Biosafety Inspection Report to the PI, and this report should be available to laboratory personnel. PIs may also perform their own self-inspection using the biosafety inspection checklist.

5. All personnel in the lab should fill out a lab-specific biosafety training checklist and keep copies available.

6. Each PI should maintain a biological inventory − of all biological organisms that may be present in the laboratory, including known hazards for each organism. Biological safety data sheets or fact sheets provided by the manufacturer should also be provided when available.

7. Personnel who work with human blood, body fluids, tissues, cell lines, or any unfixed human tissue must be provided Hepatitis B Virus vaccinations free of charge. If personnel decline the vaccination, the HBV vaccine declination forms must be signed and maintained.

8. A list of all emergency laboratory personnel and contact information should be recorded and made available.

9. Standard Operating Procedures relevant to the laboratory must be written and made available to lab personnel. Examples include the BSL-2SOP, Biosafety Cabinet SOP, and Autoclave Operation SOP. Other SOPs may be developed for emergency spill clean-up, biohazardous waste disposal, accident/incident Reporting, transporting biological materials, safe handling of sharps, post-exposure medical surveillance, or use of specific equipment in the lab.

10. A spill kit should be kept in each laboratory where work with biohazards is conducted. Spill kit contents should include: disinfectant (bleach or organism-specific disinfectant), paper towels, gloves, autoclave bags, sharps container, forceps, and a broom and dustpan. A wrap-around lab coat and safety glasses should also be available. Note: A 1:10 dilution of household bleach and water, prepared fresh daily, is effective in most situations. Contact EHS Biosafety Biosecurity at 480-965-1823 for more information about the selection of disinfectants, particularly for any organisms suspected of being atypical in their sensitivity to disinfectants.

Please keep these materials in your laboratory and have them available to all lab personnel. Annual biosafety inspections will require the laboratory to produce these documents in accordance with federal regulations. If you have any questions about your Lab-Specific Biosafety Manual, please contact Biosafety.